4,813 research outputs found
On the Slope-Aspect Correction of Multispectral Scanner Data
The effects of topography on the radiometric properties of multispectral scanner (MSS) data are examined in the context of the remote sensing of forests in mountainous regions. The two test areas considered for this study are located in the coastal mountains of British Columbia, one at the Anderson River near Boston Bar and the other at Gun Lake near Bralorne. The predominant forest type at the former site is Douglas fir, whereas forest types at the latter site are primarily lodgepole pine and ponderosa pine. Both regions have rugged topography, with elevations ranging from 275 to 1500 metres above sea level at Anderson River and from 670 to 1990 metres above sea level at Gun Lake.
Lambertian and non-Lambertian illumination corrections are formulated, taking into account atmospheric effects as well as topographic variations. Terrain slope and aspect values are determined from a digital elevation model and atmospheric parameters are obtained from a model atmosphere computation for the solar angles and spectral bands of interest. In the Lambertian approximation, if sky irradiance and atmospheric path radiance are neglected, one is left with a cosine correction analogous to the one which has been used extensively to carry out illumination transformations of images of horizontal terrain. However, this extension of the simple cosine correction to the case of sloped terrain is shown to be inadequate, especially for larger angles of incidence.
Attempts are also made to remove the effect of topography by means of semi-empirical functions primarily based on cosines of the incident and reflected illumination angles. In this vein, correlations and linear regressions between topographic parameters (such as elevation, slope, aspect, incidence angle, reflection angle) and MSS radiance values are investigated for the different forest types under consideration at each site.
The analysis encompasses multitemporal Landsat MSS data at a resolution of 50 metres and 11 channel airborne MSS at resolutions of 20 and 50 metres. Slope aspect correction algorithms for both of these types of data are implemented in software on the image analysis system at the Canada Centre for Remote Sensing. Geometric rectification is also a prerequisite in order to relate image geometry to the map coordinates on which the digital terrain data are based. A special technique involving flight line modelling is used to accomplish this in the case of aircraft data since prior knowledge of the terrain elevation is needed for each image pixel in order to establish an undistorted transformation.
Feature selection based on divergence criteria indicates that terrain parameters compare favourably with the MSS data in terms of ability to distinguish between forest classes. However, maximum likelihood classification results for MSS data, corrected for slope-aspect effects using a variety of functions, show little or no significant improvement over results obtained using uncorrected data. This outcome is discussed with a view to achieving a better understanding of both the physical principles and the image processing methodologies involved
CCRS proposal for evaluating LANDSAT-4 MSS and TM data
The measurement of registration errors in LANDSAT MSS data is discussed as well as the development of a revised algorithm for the radiometric calibration of TM data and the production of a geocoded TM image
Evolution of genes and repeats in the Nimrod superfamily
The recently identified Nimrod superfamily is characterized by the presence of a special type of EGF repeat, the NIM repeat, located right after a typical CCXGY/W amino acid motif. On the basis of structural features, nimrod genes can be divided into three types. The proteins encoded by Draper-type genes have an EMI domain at the N-terminal part and only one copy of the NIM motif, followed by a variable number of EGF-like repeats. The products of Nimrod B-type and Nimrod C-type genes (including the eater gene) have different kinds of N-terminal domains, and lack EGF-like repeats but contain a variable number of NIM repeats. Draper and Nimrod C-type (but not Nimrod B-type) proteins carry a transmembrane domain. Several members of the superfamily were claimed to function as receptors in phagocytosis and/or binding of bacteria, which indicates an important role in the cellular immunity and the elimination of apoptotic cells. In this paper, the evolution of the Nimrod superfamily is studied with various methods on the level of genes and repeats. A hypothesis is presented in which the NIM repeat, along with the EMI domain, emerged by structural reorganizations at the end of an EGF-like repeat chain, suggesting a mechanism for the formation of novel types of repeats. The analyses revealed diverse evolutionary patterns in the sequences containing multiple NIM repeats. Although in the Nimrod B and Nimrod C proteins show characteristics of independent evolution, many internal NIM repeats in Eater sequences seem to have undergone concerted evolution. An analysis of the nimrod genes has been performed using phylogenetic and other methods and an evolutionary scenario of the origin and diversification of the Nimrod superfamily is proposed. Our study presents an intriguing example how the evolution of multigene families may contribute to the complexity of the innate immune response
In search of lost introns
Many fundamental questions concerning the emergence and subsequent evolution
of eukaryotic exon-intron organization are still unsettled. Genome-scale
comparative studies, which can shed light on crucial aspects of eukaryotic
evolution, require adequate computational tools.
We describe novel computational methods for studying spliceosomal intron
evolution. Our goal is to give a reliable characterization of the dynamics of
intron evolution. Our algorithmic innovations address the identification of
orthologous introns, and the likelihood-based analysis of intron data. We
discuss a compression method for the evaluation of the likelihood function,
which is noteworthy for phylogenetic likelihood problems in general. We prove
that after preprocessing time, subsequent evaluations take time almost surely in the Yule-Harding random model of -taxon
phylogenies, where is the input sequence length.
We illustrate the practicality of our methods by compiling and analyzing a
data set involving 18 eukaryotes, more than in any other study to date. The
study yields the surprising result that ancestral eukaryotes were fairly
intron-rich. For example, the bilaterian ancestor is estimated to have had more
than 90% as many introns as vertebrates do now
Evolutionary Toggling of Vpx/Vpr Specificity Results in Divergent Recognition of the Restriction Factor SAMHD1
SAMHD1 is a host restriction factor that blocks the ability of lentiviruses such as HIV-1 to undergo reverse transcription in myeloid cells and resting T-cells. This restriction is alleviated by expression of the lentiviral accessory proteins Vpx and Vpr (Vpx/Vpr), which target SAMHD1 for proteasome-mediated degradation. However, the precise determinants within SAMHD1 for recognition by Vpx/Vpr remain unclear. Here we show that evolution of Vpx/Vpr in primate lentiviruses has caused the interface between SAMHD1 and Vpx/Vpr to alter during primate lentiviral evolution. Using multiple HIV-2 and SIV Vpx proteins, we show that Vpx from the HIV-2 and SIVmac lineage, but not Vpx from the SIVmnd2 and SIVrcm lineage, require the C-terminus of SAMHD1 for interaction, ubiquitylation, and degradation. On the other hand, the N-terminus of SAMHD1 governs interactions with Vpx from SIVmnd2 and SIVrcm, but has little effect on Vpx from HIV-2 and SIVmac. Furthermore, we show here that this difference in SAMHD1 recognition is evolutionarily dynamic, with the importance of the N- and C-terminus for interaction of SAMHD1 with Vpx and Vpr toggling during lentiviral evolution. We present a model to explain how the head-to-tail conformation of SAMHD1 proteins favors toggling of the interaction sites by Vpx/Vpr during this virus-host arms race. Such drastic functional divergence within a lentiviral protein highlights a novel plasticity in the evolutionary dynamics of viral antagonists for restriction factors during lentiviral adaptation to its hosts. © 2013 Fregoso et al
Disturbance and Predictability of Flowering Patterns in Bird-Pollinated Cloud Forest Plants
The distribution and flowering patterns of hummingbirdâpollinated plants were compared from July 1981 to June 1983 in three patch types in cloud forest at Monteverde, Costa Rica. Study plots were: (1) four recent, large (1100â2500 m2) disturbances ("cutovers") produced by cutting vegetation, (2) six recent, smaller (200â600 m2) disturbances caused by treefalls, and (3) four plots (1600â1800 m2) of canopied forest. Based on published literature dealing with communities that characterize different regimes of disturbance, we tested one assumption and two hypotheses. Assumption: Plant species composition differs among the three patch types. Hypothesis 1: Phenotypic specialization by plants for coâevolved interactions with hummingbirds will be lowest in large gaps, highest in forest, and intermediate in treefalls. Hypothesis 2: Predictability of flowering phenologies and nectar production will be lowest in large gaps, highest in forest, intermediate in treefalls. Neither the assumption nor the hypotheses were supported by the results. The patch mosaic in this cloud forest was not associated with major differences in species composition of birdâpollinated plants. Most species studied were selfâcompatible. Most abundant in cutovers were species with long corollas, relatively specialized for attracting longâbilled hummingbirds. Species with short corollas, which can be visited by many hummingbird species and some insects, were most abundant in treefalls and forest. Variation in phenological patterns showed no consistent trends among patch types. Predictability of flower and nectar production tended to be greatest in treefalls, which are foci of concentrated flowering activity by all species. Discrepancies between our results and previous studies can be ascribed to two facts. (1) Much of the literature dealing with ecological consequences of disturbance has dealt with largeâscale anthropogenic disturbances such as old fields of the eastern USA, whereas we studied small, natural, or quasiânatural disturbances. (2) Studies of forest disturbance have focused on the tree layer, whereas we studied the understory herbs, shrubs, and epiphytes. Natural disturbance usually involves death and replacement of one or more trees, whereas individuals of other life forms may persist through the disturbance
Establishing the precise evolutionary history of a gene improves prediction of disease-causing missense mutations
PURPOSE: Predicting the phenotypic effects of mutations has become an important application in clinical genetic diagnostics. Computational tools evaluate the behavior of the variant over evolutionary time and assume that variations seen during the course of evolution are probably benign in humans. However, current tools do not take into account orthologous/paralogous relationships. Paralogs have dramatically different roles in Mendelian diseases. For example, whereas inactivating mutations in the NPC1 gene cause the neurodegenerative disorder Niemann-Pick C, inactivating mutations in its paralog NPC1L1 are not disease-causing and, moreover, are implicated in protection from coronary heart disease. METHODS: We identified major events in NPC1 evolution and revealed and compared orthologs and paralogs of the human NPC1 gene through phylogenetic and protein sequence analyses. We predicted whether an amino acid substitution affects protein function by reducing the organismâs fitness. RESULTS: Removing the paralogs and distant homologs improved the overall performance of categorizing disease-causing and benign amino acid substitutions. CONCLUSION: The results show that a thorough evolutionary analysis followed by identification of orthologs improves the accuracy in predicting disease-causing missense mutations. We anticipate that this approach will be used as a reference in the interpretation of variants in other genetic diseases as well. Genet Med 18 10, 1029â1036
Fuzzy species limits in Mediterranean gorgonians (Cnidaria, Octocorallia): inferences on speciation processes
The study of the interplay between speciation and hybridization is of primary importance in evolutionary biology. Octocorals are ecologically important species whose shallow phylogenetic relationships often remain to be studied. In the Mediterranean Sea, three congeneric octocorals can be observed in sympatry: Eunicella verrucosa, Eunicella cavolini and Eunicella singularis. They display morphological differences and E.singularis hosts photosynthetic Symbiodinium, contrary to the two other species. Two nuclear sequence markers were used to study speciation and gene flow between these species, through network analysis and Approximate Bayesian Computation (ABC). Shared sequences indicated the possibility of hybridization or incomplete lineage sorting. According to ABC, a scenario of gene flow through secondary contact was the best model to explain these results. At the intraspecific level, neither geographical nor ecological isolation corresponded to distinct genetic lineages in E.cavolini. These results are discussed in the light of the potential role of ecology and genetic incompatibilities in the persistence of species limits.French National Research Agency (ANR) program Adacni (ANR) [ANR-12-ADAP-0016]CNRSHubert Curien 'Tassili' program [12MDU853]CCMAR Strategic Plan from Fundacao para a Ciencia e a Tecnologia-FCT [PEst-C/MAR/LA0015/2011,FEDERinfo:eu-repo/semantics/publishedVersio
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